Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Details-enriched characterization of mobile populations. Morphological information and facts from photos can increase to the richness of flow cytometry information. For example, the figure shows an if not typical apoptosis assay employing Annexin V and PI, incorporating cell imaging to characterize cells in Just about every inhabitants to expose morphologically unique functions. These insights couldn't are received from multiplex staining by itself.
Whether you’re a rookie or a specialist, check out our resources to advance your exploration and expertise in flow cytometry.
Combine acoustic focusing engineering with superior-parameter Examination for adaptable spectral unmixing and traditional workflows.
Uncover new insights into your experimental data by visualizing populations utilizing blended fluorescence and morphology knowledge. Our computer software interface wizards permit downsampling, concatenation (merged data files) and dimensionality resources which are developed in to the application for an intuitive, streamlined knowledge Evaluation expertise.
"A picture isn't really quite value 1000 dots, however it seriously helps you to see pictures. It is excellent to find out visuals that correlate with staining. For instance, AnnexinV-good cells which are morphologically various than balanced cells, or CD14+ monocytes which are larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, an experienced person has gated singlets confidently. Immediately after evaluating the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of a lot more than four% aggregates.
Pair your Attune flow cytometer by having an autosampler for greater workflow effectiveness, and increase robotic integration for optimum throughput.
Reliable picture high-quality even at substantial throughput Acoustic focusing as well as a superior-pace digital camera combine to read more graphic these T cells regularly at small or superior flow rates. Conveniently change concentrate and digicam options to fulfill experimental prerequisites.
The Attune NxT cytometer may be the reputable workhorse instrument your lab can rely on for constant and dependable general performance. The Attune CytPix model encompasses a significant-pace brightfield digicam and AI-driven picture Investigation software program, with latest updates including device Finding out applications and an intuitive user interface, enabling Increased details precision, accuracy, and speedier insights into sample biology.
Perhaps most significantly, these activities have cells of Plainly different phenotypes which could lead to incorrect conclusions about double optimistic gatherings (particularly in uncommon populations).
Audio waves permit specific cell alignment, leading to Fantastic details high quality and ~10X a lot quicker sample flow charges.
We Earlier shown the power of imaging Vehicle-T/Ramos mobile interactions. Permit’s examine just the populace of biggest desire, the double beneficial situations, to learn more. We can now use prolonged morphological parameters (below, circularity and skewness of depth) to even more analyze the attributes of those populations and refine gating on these occasions, growing facts robustness.
Leverage the power of Highly developed technologies and innovation to accelerate your discoveries. Belief while in the Attune Flow Cytometer loved ones to provide the trustworthiness, speed, and precision that established your operate apart.
Adaptable payment approach from Agilent Money Remedies help you receive the vital instrumentation you require for quick job, or to grow your lab.
Gating on these extended parameters allows you to promptly and properly determine populations of interest and ensure gating method with little or no manual evaluation. The Attune software features usage of point out-of-the-art machine Studying Evaluation equipment in just an expertly created person interface, which makes it exceptionally convenient to use.
The Attune Xenith cytometer is actually a superior-parameter spectral analyzer, delivering both of those spectral unmixing and traditional flow capabilities for extensive and multipurpose cell Assessment.
Agilent Price Promise ensures you at the very least ten years of instrument use through the date you purchase. If not, we will credit you Along with the residual worth of the technique towards an upgraded model.
What appeared to be a homogeneous population is definitely additional assorted—an insight that needs to be regarded when interpreting effects.